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Assay method using high performance liquid chromatography with diode array detection was developed and validated for the determination of chromones: prim-Oglucosylcimifugin, cimifugin, 4’-O-β-D-glucosyl-5-O-methylisamminol. Chromatographic method for the determination of chromones was validated for specificity, linearity, accuracy, repeatability and intermediate precision. The optimal conditions for the extraction of chromones from the  Saposhnikoviae divaricatae radices  were selected. It was found that these substances are better extracted with 50% ethanol using ultrasonic extraction at room temperature. The quantity of chromones in  Saposhnikoviae Radix  of the flora of Buryatia was also determined.

Development of assay method by HPLC-DAD for the quantitative determination of chromones in Saposhnikovia divaricata radices and its validation