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Nutrition addition in Microbial Enhanced Oil Recovery (MEOR) application is one of the methods used to increase oil production. Nutrition injection in MEOR application must be done carefully because the lack of proper nutrition addition can induce biofilm and biocorrosion formation. The composition of biostimulation medium for optimizing beneficial indigenous bacterial growth in Microbial Enhanced Oil Recovery (MEOR) was evaluated using Response Surface Methodology (RSM) with Central Composite Design (CCD). Three main factors for the medium composition were molasses, Diammonium Phosphate (DAP), and NPK. The RSM was used to know the most effective medium composition towards biofilm production and strength that high potential leads to biocorrosion. Molasses, NPK, and diammonium phosphate (DAP) are utilized as carbon, nitrogen, and phosphate supplementary substrate in Brine water as basal medium. Molasses concentration was 0%-10% while DAP and NPK was 0%-0.5%. Both aerobic and anaerobe sessile bacteria, as well as acid producing bacteria, were enumerated by total plate and turbidity methods. Statistical analysis with α=0.05 proves molasses give significance effect to biofilm strength and sessile anaerobic bacteria. DAP gives significance effect to biofilm strength and sessile aerobic and anaerobic bacteria. NPK give significance effect to sessile anaerobic bacteria. Molasses addition decrease brine pH by microbial activity, make the carbon steel coupon ST-37 corrosion rate increased. Meanwhile, the addition of DAP increased the pH of brine medium and decrease the corrosion rate.

Effects of nutrient composition on the formation of biofilm and biocorrosion in MEOR biostimulation medium based on response surface methodology