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Background: 4Aminoantipyrine (AAP) is widely used in the pharmaceutical industry, in biochemical experiments and in environmental monitoring. AAP as an aromatic pollutant in the environment poses a great threat to human health. Methods: A spectrophotometric, A Sensitive, simple accurate and inexpensive way has been proposed for the estimation of Tetracycline The way is based on the oxidation reaction coupling of drug Tetracycline by 4-amenoantypyren (4AAP) in basic medium, and then reacts by Selenium dioxide (SeO2) to give a complex colored at temperature room which produce a product having maximum absorption at 553 nm. Results: The data find that the order of addition, Tetracycline –base - Selenium dioxide (SeO2) -4- AAP, gave maximum absorbance and sensitivity, the good base was (0.1N) sodium hydroxide needful for developing the colored product and raise its stability. That complex, at 25°C, gave a better sensitivity and was selected for further utilize in this study. Under optimum experimental conditions obtained via the linearity of the constructed calibration curve was in the range of 1-20 mg. L of Tetracycline drug with the detection limit of 1.12 x 10 -5 mg/L and molar absorptivity was 3.915 x 10 3  L/mol.cm. Conclusion: The used way seemed simple, fast, precision and accurate. In adding, it is applicable for the assay of the Tetracycline in study in many amount forms and the data are in best concord with those obtained in previous studies.

Role of Selenium dioxide in Spectrophotometric determination of Tetracycline in pure and pharmaceutical formulations