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The unprecedented reduction in the availability of fossil fuels requires the research community to find safe, clean, renewable and sustainable energy sources. One promising source is microalgae biofuel, which can reduce environmental problems and energy crises due to its closed carbon cycle. Selecting the right strain and optimizing lipid productivity are very important for the success of the biodiesel conversion process economically. In this study, the lipid extraction process from  Aphanothece sp . dry biomass which was previously grown in photobioreactor systems with atmospheric carbon dioxide feed inputs was carried out using the ultrasound method, with variations of solvent systems. The maximum lipid extract yield of about 40.79 ± 0.76 percent DW, w/w was obtained for the dichloromethane/methanol ratio of 1:2 (v/v).

Lipid extraction from Aphanothece sp. using ultrasounds