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The purpose of this research is to describe Senior High School Biology teachers perception towards implementation of 2013 revised curriculum according to school area zone in Gunungkidul Regency, including graduates competencies aspect, process aspect, assessment aspect and follow up aspect. It is quantitative descriptive research. Research subject consisted of 20 Biology teachers from twelve senior high schools in Gunungkidul Regency. The data in this study were collected using a questionnaire, interview and documentation. Validity is determined by construct validity ( expert judgment ) and skalo program. Reliability is calculated by Kuder Richardson 20. The technique of analysis through descriptive analysis. The results indicate that different perception occur in three zones. Teachers perception of four aspects as follows: (1) Northern Zone was good in three aspects and still poor in process aspect (2) Middle Zone was excellent in four aspects (3) Southern Zone was good in four aspects. There was concluded that Biology teachers in Gunungkidul Regency have different perception in four aspects of 2013 revised curriculum.