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A lab built semi-automated stopped-flow system was employed for spectrophotometric determination of nitrite in different water samples. The constructed system consists two microcontrollers (Arduinos) supplied with a home- made software programs. The first one was UNO type used to manipulates a home-made injection-pump stepper-motor type and to control the reaction-time in the flow cell. The other one was Mega type which was used as data-logger to record the results as stopped-flow peak by using Microsoft Excel 2010 program. The linearity was in the range (0.05 – 0.5) µg/ml with regression coefficient of 0.9960. The detection limit was 0.025 µg/ml and the samples can be analyzed at rate exceeding 45 samples/h. The concentration of nitrite in the different water samples were in the range (0.062 – 0.326 ) µg/ml.

Lab – Built semi- automated Stop – flow System for Spectrophotometric Determination of Nitrite in Different water Samples