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The present study aimed to the phenotypic and genetic detection of some virulence factors in Methicillin resistant  Staphylococcus aureus  (MRSA) isolated from burn patients. from a total of 38 isolates of  Staphylococcus aureus , 15(39.4%) isolates of MRSA were collected from the patients in Diwaniyah Teaching Hospital during the period from June to October, 2018 in AL-Diwaniyah City. Methicillin resistant  Staphylococcus aureus  (MRSA) was detected by using chrom agar  MRSA  and polymerase chain reaction (PCR), 15 isolates (39.4%) reported resistance to methicillin antibiotic.In addition, phenotypic detection of the ability of MRSA to produce the ß-lactamase enzymes using rapid iodometric method and its ability to form the biofilm was determined by using the tube method, The results showed 15(100%) MRSA isolates were able to produce ß-lactamase enzymes and 14 (93.3%) MRSA isolates were able to biofilm production. On the other hand, All MRSA isolates were tested by using Polymerase Chain Reaction (PCR) to detection of some virulence factors genes, which include  coa  gene encoding production of coagulase,  icaA  gene encoding for biofilm production and  blaZ  gene encoding for the ß-lactamase enzymes. PCR results showed that MRSA isolates were posses  blaZ  gene (100%),  icaA  gene (93.3%) and  coa  gene (86.6%).

The phenotypic and genetic characterization of some virulence factors in MRSA isolated from burn patients