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This study analyze the prevalence of  bla CTX-M  containing  Klebsiella pneumoniae . During the period from October, 2017 to the end of January, 2018, a total of one hundred swab samples were collected from environment of Al-Hillah Teaching Hospital / Hillah city. Thirteen (13%) isolates were identified as  K.pneumoniae . All  K.pneumoniae  isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Higher resistance rates were observed for penicillin antibiotics (ampicillin and cloxacillin) with resistance rate of (84.61%) and (69.23%), respectively. Extended spectrum beta-lactamase (ESBL) production was assayed phenotypically using disk combination method. Five (38%) isolates were screen-positive. Carbapenem resistance was detected in 2 isolates of  K.pneumoniae , these were checked further by Polymerase Chain Reaction (PCR) method for the presence of  bla CTX-M  gene, 1 (50%) isolate gave positive result.

journal of physics. conference series

Molecular detection of CTX-M extended spectrum beta-lactamase among carbapenem- resistant Klebsiella pneumoniae from Al-Hillah Teaching Hospital environment, Babylon Province, Iraq