Open AccessComputational analyses reveal spatial relationships between nuclear pore complexes and specific lamins
Author(s) -
Mark Kittisopikul,
Takeshi Shimi,
Meltem Tatli,
Joseph Tran,
Yixian Zheng,
Ohad Medalia,
Khuloud Jaqaman,
Stephen A. Adam,
Robert D. Goldman
Publication year - 2021
Publication title -
the journal of cell biology/the journal of cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.414
H-Index - 380
eISSN - 1540-8140
pISSN - 0021-9525
DOI - 10.1083/jcb.202007082
Subject(s) - lamin , nucleoporin , nuclear pore , biology , nuclear lamina , lmna , microbiology and biotechnology , invadopodia , gene isoform , cell nucleus , nucleus , nuclear protein , genetics , nuclear transport , transcription factor , gene , cancer , cancer cell
Nuclear lamin isoforms form fibrous meshworks associated with nuclear pore complexes (NPCs). Using datasets prepared from subpixel and segmentation analyses of 3D–structured illumination microscopy images of WT and lamin isoform knockout mouse embryo fibroblasts, we determined with high precision the spatial association of NPCs with specific lamin isoform fibers. These relationships are retained in the enlarged lamin meshworks of Lmna−/− and Lmnb1−/− fibroblast nuclei. Cryo-ET observations reveal that the lamin filaments composing the fibers contact the nucleoplasmic ring of NPCs. Knockdown of the ring-associated nucleoporin ELYS induces NPC clusters that exclude lamin A/C fibers but include LB1 and LB2 fibers. Knockdown of the nucleoporin TPR or NUP153 alters the arrangement of lamin fibers and NPCs. Evidence that the number of NPCs is regulated by specific lamin isoforms is presented. Overall the results demonstrate that lamin isoforms and nucleoporins act together to maintain the normal organization of lamin meshworks and NPCs within the nuclear envelope.