Open AccessHematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1
Author(s) -
Thomas Bessy,
Adrian Candelas,
Benoît Souquet,
Khansa Saadallah,
Alexandre Schaeffer,
Benoît Vianay,
Damien Cuvelier,
Samy Gobaa,
Cecilia Nakid-Cordero,
Julien Lion,
Raphaël Borie,
Nuala Mooney,
Thierry Jaffredo,
Jérôme Larghero,
Laurent Blanchoin,
Lionel Faivre,
Stéphane Brunet,
Manuel Théry
Publication year - 2021
Publication title -
the journal of cell biology/the journal of cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.414
H-Index - 380
eISSN - 1540-8140
pISSN - 0021-9525
DOI - 10.1083/jcb.202005085
Subject(s) - microbiology and biotechnology , stromal cell , biology , progenitor cell , haematopoiesis , bone marrow , centrosome , cell polarity , stem cell , cell , immunology , cancer research , cell cycle , genetics
The fate of hematopoietic stem and progenitor cells (HSPCs) is regulated by their interaction with stromal cells in the bone marrow. However, the cellular mechanisms regulating HSPC interaction with these cells and their potential impact on HSPC polarity are still poorly understood. Here we evaluated the impact of cell–cell contacts with osteoblasts or endothelial cells on the polarity of HSPC. We found that an HSPC can form a discrete contact site that leads to the extensive polarization of its cytoskeleton architecture. Notably, the centrosome was located in proximity to the contact site. The capacity of HSPCs to polarize in contact with stromal cells of the bone marrow appeared to be specific, as it was not observed in primary lymphoid or myeloid cells or in HSPCs in contact with skin fibroblasts. The receptors ICAM, VCAM, and SDF1 were identified in the polarizing contact. Only SDF1 was independently capable of inducing the polarization of the centrosome–microtubule network.