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Effect of prooxidants on mitochondrail permeability transition and cell death in Ehrlich ascites tumour cells
Author(s) -
Teplova Vera V.,
Kudrjavtsev Andrew A.,
Odinokova Irina V.,
Evtodienko Yuri V.,
Saris NilsErik L.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202882
Subject(s) - mitochondrial permeability transition pore , programmed cell death , mitochondrion , apoptosis , menadione , chemistry , incubation , cell , membrane permeability , membrane potential , biophysics , permeability (electromagnetism) , ehrlich ascites , microbiology and biotechnology , membrane , biology , biochemistry , cancer research , tumor cells , enzyme
Ca2+ retention in mitochondria, opening of the Cysclosporin A‐ sensitive permeability transition pore and cell death were studied in Ehrlich ascites tumour cells in the presence of different prooxidants. Low concentrations (1‐20 μM) of the prooxidants (menadione, cumenehydroperoxide, t‐butylhydroperoxide) induced pore‐opening in permeabilized cells at threshold Ca2+ load. Incubation of cells with low concentrations of prooxidants was able to induce cell cycle disturbance and cell death. Under the prooxidant effect, mitochondrial membrane potential drop and Ca2+ retention decrease in mitochondria were found to precede death of Ehrlich ascites tumour cells.