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Inductions of immediate early genes (IEGs) and ref‐1 by human chorionic gonadotropin in murine Leydig cell line (MA‐10)
Author(s) -
Suzuki Syoji,
Nagaya Takashi,
Suganuma Nobuhiko,
Tomoda Yutaka,
Seo Hisao
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800201242
Subject(s) - human chorionic gonadotropin , leydig cell , gonadotropin , medicine , gene , endocrinology , andrology , biology , microbiology and biotechnology , genetics , hormone , luteinizing hormone
The effect of human chorionic gonadotropin (hCG) on the expression of immediate early genes (IEGs) including all members of fos and jun family, and c‐myc was studied using mouse Leydig cell line (MA‐10 cells) by Northern blot analyses. In addition, the induction of ref‐1 which enhances DNA binding of fos/jun proteins was also analyzed. HCG induced a rapid and transient expression of c‐fos, fosB, c‐jun, junB, junD and c‐myc with a peak at 30 min to 1h. In contrast, induction of fra‐1 mRNA was delayed with a peak at 3 hr. However, fra‐2 mRNA was immediately increased by hCG with a peak at 1 h. The ref‐1 mRNA was expressed before the stimulation and its level was not altered by hCG at least for 8 hr. The differential induction of IEGs and continuous expression of ref‐1 mRNA suggest an important role of their gene products on the regulation of Leydig cell function by hCG.