Premium
Inhibition of the proliferative effect of transforming growth factor‐α by c‐myc antisense DNA in human ovarian cancer cells
Author(s) -
Park HaeYoung
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204831
Subject(s) - microbiology and biotechnology , transforming growth factor , cancer research , tgf alpha , ovarian cancer , cell growth , biology , oligonucleotide , gene , dna synthesis , alpha (finance) , cell culture , transforming growth factor beta , growth factor , dna , cancer , medicine , biochemistry , genetics , receptor , nursing , patient satisfaction , construct validity
In search of critical genes associated with the mechanism of transforming growth factor‐α (TGFα) action in human ovarian cancer, it was found that TGFα stimulates c‐rnyc gene expression in human ovarian NIH:OVCAR‐3. The role of c‐myc in TGFα‐stimulated growth of NIH:OVCAR‐3 cells was examined by the use of the synthetic antisense‐myc phosphorothioate oligonucleotide (OPT). Prior exposure of NIH:OVCAR‐3 cells to an antisense‐myc OPT inhibited TGFα‐stimulated cell growth and DNA syntbesis in a dose‐dependent and sequence‐spocific manner over 4 days. c‐Myc protein expression was down‐regulated in the antisense‐myc treated cells. These results demonstrate both the specific and durable effects of the antisense‐myc OPT. Furthermore, the results suggest a role for c‐myc in TGFα‐stimulated cell proliferation.