Premium
Identification, purification, and partial characterization of a factor from rabbit serum that inhibits prolactin secretion by pituitary cells cultured in vitro
Author(s) -
Giacomelli Sabrina,
Cheng C. Yan,
Leone Maria Grazia,
Palmery Maura,
Silvestrini Bruno
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203671
Subject(s) - prolactin , in vitro , secretion , endocrinology , medicine , pituitary gland , endogeny , chemistry , biology , hormone , biochemistry
A biological factor that inhibited prolactin secretion by pituitary cells cultured in vitro was identified, purified, and partially characterized from normal rabbit serum. This biological factor was also found to potentiate dopamine‐mediated aortic contraction using rabbit aortic strips in vitro. Following SDS‐PAGE, this factor displayed an apparent Mr of 17 kDa, which is different from the Mr of most known endogenous factors having an inhibiting activity on pituitary prolactin secretion, suggesting that this may be a yet‐to‐be identified novel molecule.