Open AccessDNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.
Author(s) -
Irene L. Andrulis,
Louis Siminovitch
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.9.5724
Subject(s) - chinese hamster ovary cell , asparagine synthetase , biology , asparagine , gene , microbiology and biotechnology , mutant , beta (programming language) , phenotype , genetics , dna , transfection , ovary , cell culture , amino acid , computer science , programming language
Cell lines that have high levels of resistance to beta-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in two steps from Chinese hamster ovary cells. Resistance to beta-aspartylhydroxmate was transferred into sensitive cells by using total genomic DNA derived from the dominant two-step mutants. The surviving colonies were characterized as transferants on the basis of transfer frequency, degree of resistance to beta-aspartylhydroxamate, increased level of asparagine synthetase activity, expression of the donor form of asparagine synthetase, codominance in hybrids, and instability of the phenotype in the absence of selection.