Open AccessThe mouse brain after foot shock in four dimensions: Temporal dynamics at a single-cell resolution
Author(s) -
Valeria Bonapersona,
Heike Schuler,
Ruth Damsteegt,
Youri Adolfs,
R. Jeroen Pasterkamp,
Martijn P. van den Heuvel,
Marian Joëls,
R. Angela Sarabdjitsingh
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2114002119
Subject(s) - neuroscience , mesoscale meteorology , atlas (anatomy) , computer science , pipeline (software) , temporal resolution , shock (circulatory) , brain mapping , biology , anatomy , physics , medicine , optics , meteorology , programming language
Significance Acute stress involves the majority of brain areas, which can be sequentially organized in functional brain networks as shown by our study with foot shock in mice. We used whole-brain microscopy to investigate different spatial resolutions over time. From mesoscale region–based analyses, we identified the order of activation of brain areas. With single-cell analyses, we analyzed shifts in activation over time within small nuclei—a result impossible to achieve with functional MRI’s resolution. These findings required the development of a four-dimensional (4D) analytical pipeline, which is made available as an R package. This “atlas” of foot shock can be visualized in 4D in our interactive web portal.