Open AccessLinking function to global and local dynamics in an elevator-type transporter
Author(s) -
Didar Ciftci,
Chloé Martens,
Vishnu G Ghani,
Scott C. Blanchard,
Gerard H. M. Huysmans,
Olga Boudker
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2025520118
Subject(s) - biophysics , substrate (aquarium) , transporter , elevator , membrane , chemistry , molecule , conformational change , molecular dynamics , transport protein , chemical physics , biochemistry , materials science , computational chemistry , biology , ecology , organic chemistry , composite material , gene
Significance Structural snapshots of membrane transporters show that they cycle through several conformational states to bring substrates across the membrane. The rates of these molecular motions determine the activity of the transporters. In this work, we directly compare real-time single-molecule measurements of conformational changes and substrate transport rates in the model glutamate transporter homologue, GltPh . We couple these experiments with hydrogen–deuterium exchange mass spectrometry measurements of local protein flexibility to directly link local and global conformational dynamics with function.