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Significance Three-dimensional refinement is a critical component of cryo-EM single-particle reconstruction. In this paper, we report the development of a computational method, OPUS-SSRI, and its application to seven real cryo-EM datasets. Our data clearly demonstrated that OPUS-SSRI can improve the final resolutions and structural details in cryo-EM single-particle analysis.

Sparseness and Smoothness Regularized Imaging for improving the resolution of Cryo-EM single-particle reconstruction