Premium
Predictive value of circulating immature cell counts in peripheral blood for timing of peripheral blood progenitor cell collection after G–CSF plus chemotherapy‐induced mobilization
Author(s) -
Kozuka Teruhiko,
Ikeda Kazuma,
Teshima Takanori,
Kojima Kensuke,
Matsuo Keitaro,
Bessho Akihiro,
Sunami Kazutaka,
Hiramatsu Yasushi,
Maeda Yoshinobu,
Noguchi Toshio,
Yamamoto Kazuhiko,
Fujii Nobuharu,
Imai Toshi,
Takenaka Katsuto,
Shinagawa Katsuji,
Ishimaru Fumihiko,
Niiya Kenji,
Koide Norio,
Tanimoto Mitsune,
Harada Mine
Publication year - 2002
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2002.00218.x
Subject(s) - apheresis , cd34 , progenitor cell , medicine , white blood cell , peripheral blood , flow cytometry , peripheral , leukapheresis , blood cell , immunology , andrology , stem cell , biology , platelet , genetics
BACKGROUND : Enumeration of CD34+ cells in peripheral blood (PB) before apheresis predicts the number of CD34+ cells collected, although flow cytometric techniques used are complex and expensive. In an attempt to determine the optimal timing for peripheral blood progenitor cell (PBPC) collection, the usefulness of circulating immature cell (CIC) counts in PB was evaluated. STUDY DESIGN AND METHODS : CIC counts in PB and CD34+ cell counts in the apheresis product from 249 collections were assessed, and the relationship between these two parameters was evaluated by with the Pearson rank correlation analysis, the Fisher exact test, and the U‐test. RESULTS : CIC counts were correlated significantly with the number of CD34+ cells per kg of patient's body weight in the apheresis product (Pearson rank correlation analysis: r = 0.635, p < 0.0001). When a level of 1 × 10 9 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 1 × 10 6 CD34+ cells per kg of body weight were 75.7 and 85.5 percent, respectively. CONCLUSION : The present study strongly suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1 × 10 9 per L can be used as a good predictor for PBPC collections containing more than 1 × 10 6 CD34+ cells per kg of body weight in a single apheresis procedure.