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Application of a laser scalpel to sectioning fragile tissues prior to optical sectioning microscopy and 3D reconstruction
Author(s) -
Cookson M. J.,
Entwistle A.
Publication year - 2000
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2000.00740.x
Subject(s) - optical sectioning , microscopy , materials science , optical microscope , laser , optics , composite material , scanning electron microscope , physics
A method is described for the cutting of fragile material with a laser scalpel which minimizes damage to friable materials, making the interior of structures accessible for optical sectioning microscopy or for high resolution X‐ray microtomography followed by 3D reconstruction.