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Role of F 1 F 0 ‐ATPase in the growth of Streptococcus mutans GS5
Author(s) -
Suzuki T.,
Tagami J.,
Hanada N.
Publication year - 2000
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2000.00840.x
Subject(s) - streptococcus mutans , library science , dental research , dental treatments , medicine , clinical microbiology , family medicine , dentistry , biology , microbiology and biotechnology , genetics , bacteria , computer science
The role of F 1 F 0 ‐ATPase in Streptococcus mutans GS5 was investigated by isolating a mutant (NTS1) defective in enzyme activity by homologous recombination with a plasmid encoding the 5′ terminal fragment of the F 1 F 0 ‐ATPase β‐subunit gene. The ATPase activity of NTS1 membranes was 49% that of GS5 membranes. The lag phase of the growth curve of NTS1 was longer than that for GS5, and the lag phase of GS5 and NTS1 were prolonged by the addition of ionophore gramicidin D; at stationary phase, the turbidity of the NTS1 culture was less than that of the GS5 culture. These results suggest that S. mutans F 1 F 0 ‐ATPase contributes to the generation of a stoichiometric electrogenic gradient effectively in the lag phase.