Open AccessMapping epitopes for 20 monoclonal antibodies to CR1
Author(s) -
M W Nickells,
Richard E. Hauhart,
M. Krych,
V. Bala Subramanian,
Kathleen Geoghegan-Barek,
Henry C. Marsh,
John Atkinson
Publication year - 1998
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1998.00549.x
Subject(s) - epitope , monoclonal antibody , epitope mapping , immunology , biology , complement system , microbiology and biotechnology , complement receptor , receptor , immune system , antibody , virology , genetics
Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.
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