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Effect of calcitonin gene‐related peptide on osteoblast differentiation in an osteoblast and endothelial cell co‐culture system
Author(s) -
Bo Yang,
Yan Li,
Gang Zhang,
Tao Lei,
Yinghui Tan
Publication year - 2012
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20110562
Subject(s) - osteoblast , alkaline phosphatase , osteocalcin , calcitonin , chemistry , umbilical vein , messenger rna , microbiology and biotechnology , endocrinology , calcitonin gene related peptide , medicine , gene expression , cell culture , in vitro , andrology , biology , biochemistry , enzyme , gene , neuropeptide , receptor , genetics
We have investigated the in vitro effects and regulatory mechanism of CGRP (calcitonin gene‐related peptide) on the differentiation of OB (osteoblasts) in co‐culture with HUVEC (human umbilical vein endothelial cells). Primary human MOB (mandibular OB) and OB‐like cells (MG‐63) were either cultured directly or indirectly co‐cultured with HUVEC at a 1:1 ratio. Expression of OC (osteocalcin) was measured by ELISA, and expression of ALP (alkaline phosphatase) and collagen mRNA was measured by quantitative fluorescent PCR. For mineralization nodus, OB were stained with Alizarin Red‐S. When co‐cultured with HUVEC, expression of OC and ALP mRNA were increased in MG‐63 ( P <0.01), and the expression of OC, ALP and collagen mRNA were increased in MOB ( P <0.01 or 0.05). When treated with CGRP, OC and ALP mRNA and mineralization nodus numbers were increased in the MG‐63 co‐culture system ( P <0.01 or 0.05); OC, ALP and collagen mRNA, and mineralization nodus numbers were increased in the MOB co‐culture system ( P <0.01 or 0.05). The effect of CGRP regulation on the differentiation of OB is not only direct but also indirect, via its effect on HUVEC and stimulation of OB.