English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In both applied and basic research,  Agrobacterium -mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three  Agrobacterium tumefaciens  strains and five T-DNA origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector ("backbone") into the genome of  Arabidopsis thaliana  and  Zea mays . Launching T-DNA from the  picA  locus of the  Agrobacterium  chromosome increases the frequency of single transgene integration events and almost eliminates the presence of vector backbone sequences in transgenic plants. Along with novel  Agrobacterium  strains we have developed, our findings are useful for improving the quality of T-DNA integration events.

Generation of "backbone" free, low transgene copy plants by launching T-DNA from the Agrobacterium chromosome