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Host Langerin (CD207) is a receptor for Yersinia pestis phagocytosis and promotes dissemination
Author(s) -
Yang Kun,
Park Chae G,
Cheong Cheolho,
Bulgheresi Silvia,
Zhang Shusheng,
Zhang Pei,
He Yingxia,
Jiang Lingyu,
Huang Hongping,
Ding Honghui,
Wu Yiping,
Wang Shaogang,
Zhang Lin,
Li Anyi,
Xia Lianxu,
Bartra Sara S,
Plano Gregory V,
Skurnik Mikael,
Klena John D,
Chen Tie
Publication year - 2015
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2015.46
Subject(s) - langerin , yersinia pestis , biology , microbiology and biotechnology , c type lectin , dendritic cell , antigen , immune system , immunology , virulence , biochemistry , gene
Yersinia pestis is a Gram‐negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium‐dependent (C‐type) lectin. Furthermore, Y. pestis possesses exposed core oligosaccharides. In this study, we show that Y. pestis invades LCs and Langerin‐expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, Y. pestis propensity to invade Langerhans and Langerin‐expressing cells decreases. Moreover, the interaction of Y. pestis with Langerin‐expressing transfectants is inhibited by purified Langerin, a DC‐SIGN (DC‐specific intercellular adhesion molecule 3 grabbing nonintegrin)‐like molecule, an anti‐CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of Y. pestis to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by Y. pestis . Therefore, Langerin‐mediated binding of Y. pestis to APCs may promote its dissemination and infection.