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Chromatin‐dependent and ‐independent regulation of DNA replication origin activation in budding yeast
Author(s) -
Lõoke Marko,
Kristjuhan Kersti,
Värv Signe,
Kristjuhan Arnold
Publication year - 2013
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2012.196
Subject(s) - budding yeast , chromatin , dna replication , biology , origin recognition complex , yeast , microbiology and biotechnology , control of chromosome duplication , replication (statistics) , genetics , dna , origin of replication , eukaryotic dna replication , saccharomyces cerevisiae , virology
To elucidate the role of the chromatin environment in the regulation of replication origin activation, autonomously replicating sequences were inserted into identical locations in the budding yeast genome and their activation times in S phase determined. Chromatin‐dependent origins adopt to the firing time of the surrounding locus. In contrast, the origins containing two binding sites for Forkhead transcription factors are activated early in the S phase regardless of their location in the genome. Our results also show that genuinely late‐replicating parts of the genome can be converted into early‐replicating loci by insertion of a chromatin‐independent early replication origin, ARS607 , whereas insertion of two Forkhead‐binding sites is not sufficient for conversion.