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CO 2 exacerbates oxygen toxicity
Author(s) -
Ezraty Benjamin,
Chabalier Maïalène,
Ducret Adrien,
Maisonneuve Etienne,
Dukan Sam
Publication year - 2011
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.7
Subject(s) - reactive oxygen species , oxidative stress , guanine , oxygen , toxicity , oxygen toxicity , mutagenesis , chemistry , escherichia coli , oxidative phosphorylation , mutant , dna , dna damage , biophysics , biochemistry , biology , nucleotide , gene , organic chemistry
Reactive oxygen species (ROS) are harmful because they can oxidize biological macromolecules. We show here that atmospheric CO 2 (concentration range studied: 40–1,000 p.p.m.) increases death rates due to H 2 O 2 stress in Escherichia coli in a dose‐specific manner. This effect is correlated with an increase in H 2 O 2 ‐induced mutagenesis and, as shown by 8‐oxo‐guanine determinations in cells, DNA base oxidation rates. Moreover, the survival of mutants that are sensitive to aerobic conditions (Hpx − dps and recA fur ), presumably because of their inability to tolerate ROS, seems to depend on CO 2 concentration. Thus, CO 2 exacerbates ROS toxicity by increasing oxidative cellular lesions.