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CESTA, a positive regulator of brassinosteroid biosynthesis
Author(s) -
Poppenberger Brigitte,
Rozhon Wilfried,
Khan Mamoona,
Husar Sigrid,
Adam Gerhard,
Luschnig Christian,
Fujioka Shozo,
Sieberer Tobias
Publication year - 2011
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2011.35
Subject(s) - library science , art history , art , computer science
Brassinosteroids (BRs) are steroid hormones that are essential for the development of plants. A tight control of BR homeostasis is vital for modulating their impact on growth responses. Although it is recognized that the rapid adaptation of de novo synthesis has a key role in adjusting required BR levels, our knowledge of the mechanisms governing feedback control is limited. In this study, we identify the transcription factor CESTA as a regulator of BR biosynthesis. ces‐D was isolated in a screen of Arabidopsis mutants by BR over‐accumulation phenotypes. Loss‐of‐function analysis and the use of a dominant repressor version revealed functional overlap among CESTA and its homologues and confirmed the role of CESTA in the positive control of BR‐biosynthetic gene expression. We provide evidence that CESTA interacts with its homologue BEE1 and can directly bind to a G‐box motif in the promoter of the BR biosynthesis gene CPD . Moreover, we show that CESTA subnuclear localization is BR regulated and discuss a model, in which CESTA interplays with BEE1 to control BR biosynthesis and other BR responses.