Research article

Bio-Aq, is an Indian Ayurvedic herbomineral preparation of selected ingredients, which provides significant protection against ischaemia and hypertension. However, there is lack of information regarding the effect of Bio-Aq on the cardiac changes associated with isoproterenol (ISO)induced myocardial infarction (MI). Objectives: This manuscript reports the preventive effect of Bio-Aq on lipid profile, cardiac marker enzymes and membrane bound enzymes in ISOinduced cardiotoxic male Wistar rats. Materials and Methods: Bio-Aq (25 and 50 mg/kg) was dissolved in dimethyl sulfoxide (DMSO) administered orally as pretreatment to Wistar rats daily for 28 days. After pretreatment, rats were induced with subcutaneous injection of ISO (85 mg/kg) at an interval of 24 h for two days to produce MI. Various biochemical markers of blood and tissue origin were estimated. Results: ISO-induced rats showed a significant increase in the activities of marker enzymes in serum and there by subsequent decrease in the heart. ISO-induced rats also showed significant alterations in the activities of membrane bound ATPase in the heart of ISO-induced rats. In addition, increase in the levels of lipids in serum and heart. A rise in the levels of phospholipids (PL), LDL and VLDL and decreased HDL were also observed in the serum and decrease in the level of high density lipoprotein-cholesterol (HDL) in serum and PL levels, in the heart of ISO-induced rats was observed. Pretreatment with Bio-Aq (25 and 50 mg/kg) positively altered the activities of marker enzymes and other biochemical parameters in ISO-induced rats. Conclusion: The present study concluded that Bio-Aq posses cardioprotective effect in ISO-induced MI in rats.