Open AccessVisual Detection of Cysteine and Homocysteine
Author(s) -
Oleksandr Rusin,
Nadia N. St. Luce,
Rezik A. Agbaria,
Jorge O. Escobedo,
Shan Jiang,
Isiah M. Warner,
Fareed Dawan,
Kun Lian,
Robert M. Strongin
Publication year - 2003
Publication title -
journal of the american chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.115
H-Index - 612
eISSN - 1520-5126
pISSN - 0002-7863
DOI - 10.1021/ja036297t
Subject(s) - chemistry , cysteine , homocysteine , amino acid , derivative (finance) , fluorescein , methionine , biochemistry , fluorescence , enzyme , physics , quantum mechanics , financial economics , economics
The determination of cysteine and homocysteine levels is of great current interest for the monitoring of desease states. A new colorimetric method for the simultaneous detection of l-cysteine and l-homocysteine has been developed. A fluorescein derivative reacts with the above amino acids, producing their respective thiazolidines resulting in color changes. Interference from other amino acids and proteins is minimal.