Open AccessSimple Fluorescence Sensing Approach for Selective Detection of Fe3+ Ions: Live-Cell Imaging and Logic Gate Functioning
Author(s) -
Puthiyavalappil Rasin,
Vipin Manakkadan,
Vishnunarayanan Namboothiri Vadakkedathu Palakkeezhillam,
Jebiti Haribabu,
César Echeverría,
Anandaram Sreekanth
Publication year - 2022
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.2c03718
Subject(s) - pyrene , fluorescence , detection limit , hela , ion , amine gas treating , chemistry , selectivity , photochemistry , density functional theory , binding constant , fluorescence lifetime imaging microscopy , analytical chemistry (journal) , computational chemistry , cell , chromatography , organic chemistry , optics , physics , biochemistry , catalysis
A pyrene-based fluorescent chemosensor APSB [ N -(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe 3+ in the presence of other metal ions. Apart from this, APSB shows high selectivity and sensitivity toward Fe 3+ ion detection. The detection limit for APSB was 1.95 nM, and the binding constant ( K b ) was obtained as 8.20 × 10 5 M -1 in DMSO/water (95/5, v/v) medium. The fluorescence quantum yields for APSB and APSB-Fe 3+ were calculated as 0.035 and 0.573, respectively. The function of this fluorescent sensor APSB can be explained through the photo-induced electron transfer mechanism which was further proved by density functional theory studies. Finally, a live-cell image study of APSB in HeLa cells was also carried out to investigate the cell permeability of APSB and its efficiency for selective detection of Fe 3+ in living cells.