Open AccessComparative Study of Electrochemical Biosensors Based on Highly Efficient Mesoporous ZrO2-Ag-G-SiO2 and In2O3-G-SiO2 for Rapid Recognition of E. coli O157:H7
Author(s) -
Kamrun Nahar Fatema,
Yin Liu,
Kwang Youn Cho,
WonChun Oh
Publication year - 2020
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.0c00895
Subject(s) - biosensor , escherichia coli , mesoporous material , cyclic voltammetry , nanotechnology , reproducibility , analytical chemistry (journal) , electrochemistry , nanoparticle , materials science , chemistry , chromatography , electrode , biochemistry , gene , catalysis
Here, we reported an innovative and electrochemical biosensor for the rapid detection of Escherichia coli O157:H 7. We fabricated the mesoporous ZrO 2 -Ag-G-SiO 2 (ZAGS) and In 2 O 3 -G-SiO 2 (IGS) sensors, and cyclic voltammetry (CV) was employed to detect the bacteria. The development of these portable sensors addresses the challenges of conventional time-consuming and more expensive laboratory-based analyses. Hence, the biosensors were highly selective to detect E. coli . The sensor could recognize an individual E. coli cell in 1 μL of sample volume within 30 s. E. coli live cells tied down on sample nanoparticles worked toward the definite acquirement of E. coli . The high thickness of negative charge on the surface of E. coli cells effectively regulated the concentration of dominant part charge carriers in the mesoporous channel, allowing a continuous check of E. coli concentration in a known sample. The signal current decreased linearly, while the E. coli concentration increased from 1.0 × 10 1 to 1.0 × 10 10 CFU/mL. ZAGS and IGS biosensors could detect E. coli in the range from 10 1 to 10 10 CFU/mL. ZAGS and IGS biosensors in this investigation showed great specificity, reproducibility, stability, and selectivity and are expected to have a great impact on applications in the detection of foodborne pathogens.