High throughput surface plasmon resonance imaging method for clinical detection of presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection

Highlights• Surface Plasmon Resonance imaging is an unprecedented technology for high throughput screening of antibody profiling of CoViD19 patients. • Fingerprinting of isotypes IgM, IgG and IgA can be performed for 384 patients in one run. • Severity of the disease correlates well with the total anti-RBD of SARS-CoV-2 concentration in CoViD19 patients • Affinity equilibrium constant (K D ) of the polyclonal antibody binding was directly proportional to the off-rate (k d ) simplifying the screening. • Screening of the strength of binding of anti RBD antibodies was possible in high throughput and in one run together with the isotype analysis in the LSA SPR imager. • An affinity maturation effect was shown for patients recovering from CoViD19. • A tool is now available to test the quality of the immune reaction of individuals to SARS-CoV-2 and its mutants in vaccination programs.