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A high-quality method for one-pot biosynthesis of silver nanoparticles (AgNPs) using onion extracts as reductant and stabilizer is reported. The synthesized AgNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis), X-ray powder diffraction (XRD), and transmission electron microscopy (TEM). UV-Vis spectroscopy results showed that the AgNP absorption band was located at a peak of 397 nm in aqueous solution. Both XRD and TEM results confirmed that the AgNPs were mainly spherical with average diameters of 6.0 nm by TEM and about 5.3-10.2 nm calculated using XRD data. The ability of AgNPs to reduce charge transfer resistance was also investigated using electrochemical impedance spectroscopy. Finally, the effect of synthesized NPs on ascorbic acid signal was investigated by square wave voltammetry. The peak current of square wave voltammograms of ascorbic acid increased linearly with its concentration in the range of 0.4-450.0μM. The detection limit for ascorbic acid was 0.1μM.

journal of food and drug analysis

Green synthesis of silver nanoparticles using onion extract and their application for the preparation of a modified electrode for determination of ascorbic acid