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P2‐021: SELF‐REPORTED OMEGA‐3 SUPPLEMENT USE IS ASSOCIATED WITH LOWER BRAIN AMYLOID DEPOSITION AND HIGHER CEREBROVASCULAR CONDUCTANCE IN NON‐DEMENTED OLDER ADULTS
Author(s) -
Kaufman Carolyn,
Chavez McKyla,
Vidoni Eric D.,
Billinger Sandra A.,
Burns Jeffrey M.
Publication year - 2019
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2019.06.1243
Subject(s) - transcranial doppler , medicine , body mass index , cerebral blood flow , dementia , blood pressure , cardiology , physical therapy , disease
Background: High-fat diets are a risk factor for Alzheimer’s disease, but little is known about the mechanisms. In addition, little is known about how lipids in the blood affect brain lipids. Here, we analyzed the lipidomic signature of cerebrospinal fluid (CSF) in older adults that received an intravenous infusion of triglycerides (TG). Methods: Older adults with Alzheimer’s risk factors (n1⁄421, age 67.7 6 8.6, 38% APOE E4 carrier status) underwent a 5-hour TG or saline infusion followed 3 hours later by lumbar puncture for CSF collection on different days in crossover design. The infusion, Liposyn II, was comprised of 10% safflower oil and 10% soybean oil. Lipids in the CSF samples were extracted using dichloromethane/methanol after the addition of isotope labeled internal standards. Lipids were analyzed using the Lipidyzer platform consisting of an AB Sciex 5500MS/MS system equipped with SelexION for differential mobility spectrometry. Multiple reaction monitoring was used to target and quantify lipids in both positive and negative ionization modes with and without differential mobility spectrometry. Results: We detected around 200 lipids per sample with an average CV of 5.2% for lab QC technical replicates. The infusion increased the concentration of diacylglycerols (p1⁄40.027) and lysophosphatidylcholines (LPC) (p1⁄40.003) as a class, and reduceddihydroceramides (p1⁄40.007). Participant characteristics of sex and cognitive diagnosis did not influence lipid class concentrations; however, E4 carriers had higher free fatty acids (p1⁄40.031).UsingMetaboAnalystwe identified9 individual lipid species that changed with the infusion (paired t test, false discovery rate q < 0.1) and 4 of these species differed by E4 carrier status. Conclusions: Acute hyperlipidemia resulted in alterations in a limited number of specific classes of lipids in the CSF. APOE genotype further influenced lipid concentrations in both the fasted and hyperlipidemic state.