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IC‐P‐129: THE IMPACT OF AGE ON CELL PROLIFERATION IN HIPPOCAMPAL SUBGRANULAR ZONE IN ADULT MOUSE BRAIN
Author(s) -
Smith Karen,
Semenov Mikhail V.
Publication year - 2018
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1016/j.jalz.2018.06.2195
Subject(s) - subgranular zone , hippocampal formation , neurogenesis , hippocampus , neuroscience , neural stem cell , biology , pathology , chemistry , microbiology and biotechnology , medicine , stem cell , subventricular zone
mice (more proliferating cells). We calculated how many times the number of mice in the sampling experiments need to be increased to obtain the same statistical power as in the direct count experiment. The most popular sampling scheme of using each sixth section required 14 times more mice if coronal sections are used, 19 times more mice if sagittal sections are used, and 51 times more if transverse sections are used. Conclusions:Sampling has a profound effect on the statistical power of adult hippocampal neurogenesis rate estimates in the mouse brain. The sampling of coronal and sagittal sections provides better results than the sampling of transverse sections. This study was supported by the Janet and Edward Gilda Charitable Foundation and in part by the Department of Veterans Affairs.