Open AccessA novel method to analyze 5‐hydroxymethylcytosine in CpG sequences using maintenance DNA methyltransferase, DNMT1
Author(s) -
Takahashi Saori,
Suetake Isao,
Engelhardt Jan,
Tajima Shoji
Publication year - 2015
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2015.09.003
Subject(s) - 5 hydroxymethylcytosine , cpg site , dna demethylation , dna methylation , dnmt1 , methyltransferase , demethylation , dna , biology , computational biology , methylation , epigenetics , dna methyltransferase , 5 methylcytosine , gene , genetics , gene expression
Hydroxymethylcytosine has been shown to be involved in DNA demethylation and gene expression. Although methods to determine the position of hydroxymethylcytosine at single‐base resolution have been reported, these methods involve some difficulties. Here, we report a simple method to analyze hydroxymethylcytosine in the CpG sequence utilizing the maintenance DNA methylation activity of DNMT1, which selectively methylates hemi‐methylated but not hemi‐hydroxymethylated CpG sequences. The method enables monitoring of the dynamics of the hydroxymethylation state of a specific genome site.