Open AccessPorcine SLITRK1: Molecular cloning and characterization
Author(s) -
Larsen Knud,
Momeni Jamal,
Farajzadeh Leila,
Bendixen Christian
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.10.001
Subject(s) - gene , cloning (programming) , biology , intron , homology (biology) , molecular cloning , messenger rna , microbiology and biotechnology , amino acid , genetics , peptide sequence , computer science , programming language
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks introns, as does its human and mouse counterparts. RT‐PCR cloning revealed two SLITRK1 transcripts: a full‐length mRNA and a transcript variant that results in a truncated protein. The encoded SLITRK1 protein, consisting of 695 amino acids, displays a very high homology to human SLITRK1 (99%). The porcine SLITRK1 gene is expressed exclusively in brain tissues.