Open AccessNuclear export of the influenza virus ribonucleoprotein complex: Interaction of Hsc70 with viral proteins M1 and NS2
Author(s) -
Watanabe Ken,
Shimizu Teppei,
Noda Saiko,
Tsukahara Fujiko,
Maru Yoshiro,
Kobayashi Nobuyuki
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.07.004
Subject(s) - nuclear export signal , ribonucleoprotein , cytoplasm , heterogeneous nuclear ribonucleoprotein , microbiology and biotechnology , nuclear transport , nucleus , biology , chemistry , cell nucleus , biochemistry , rna , gene
The influenza virus replicates in the host cell nucleus, and the progeny viral ribonucleoprotein complex (vRNP) is exported to the cytoplasm prior to maturation. NS2 has a nuclear export signal that mediates the nuclear export of vRNP by the vRNP–M1–NS2 complex. We previously reported that the heat shock cognate 70 (Hsc70) protein binds to M1 protein and mediates vRNP export. However, the interactions among M1, NS2, and Hsc70 are poorly understood. In the present study, we demonstrate that Hsc70 interacts with M1 more strongly than with NS2 and competes with NS2 for M1 binding, suggesting an important role of Hsc70 in the nuclear export of vRNP.