Open AccessInhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole–imidazole polyamide, which targets an E‐box motif
Author(s) -
Taniguchi Masashi,
Fujiwara Kyoko,
Nakai Yuji,
Ozaki Toshinori,
Koshikawa Nobuko,
Toshio Kojima,
Kataba Motoaki,
Oguni Asako,
Matsuda Hiroyuki,
Yoshida Yukihiro,
Tokuhashi Yasuaki,
Fukuda Noboru,
Ueno Takahiro,
Soma Masayoshi,
Nagase Hiroki
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2014.03.004
Subject(s) - malat1 , gene knockdown , osteosarcoma , phenotype , gene , biology , cancer research , gene silencing , transcription factor , in vitro , microbiology and biotechnology , rna , long non coding rna , biochemistry
Gene amplification and/or overexpression of the transcription factor c‐MYC, which binds to the E‐box sequence (5′‐CACGTG‐3′), has been observed in many human tumors. In this study, we have designed 5 pyrrole–imidazole (PI) polyamides recognizing E‐box, and found that, among them, Myc‐6 significantly suppresses malignant phenotypes of human osteosarcoma MG63 cells both in vitro and in vivo. Intriguingly, knockdown of the putative Myc‐6 target MALAT1 encoding long noncoding RNA remarkably impaired cell growth of MG63 cells. Collectively, our present findings strongly suggest that Myc‐6 exerts its tumor‐suppressive ability at least in part through the specific down‐regulation of MALAT1 .