Open AccessDiphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide‐specific transporter
Author(s) -
Massart C.,
Giusti N.,
Beauwens R.,
Dumont J.E.,
Miot F.,
Sande J. Van
Publication year - 2014
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2013.11.007
Subject(s) - chemistry , transporter , iodide , iodine , enzyme , cell culture , biochemistry , thyroid , efflux , biology , gene , endocrinology , genetics , organic chemistry
NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O 2 .− and H 2 O 2 . Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O 2 derivatives, activated the efflux of radioiodide but not of its analog 99m TcO 4 − nor of the K + cation mimic 86 Rb + in thyroid cells, in the PCCl3 rat thyroid cell line and in COS cell lines expressing the iodide transporter NIS. Effects obtained with DPI, especially in thyroid cells, should therefore be interpreted with caution.