Open AccessThe carboxy‐terminal segment of the human LINE‐1 ORF2 protein is involved in RNA binding
Author(s) -
Piskareva Olga,
Ernst Christina,
Higgins Niamh,
Schmatchenko Vadim
Publication year - 2013
Publication title -
febs open bio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 31
ISSN - 2211-5463
DOI - 10.1016/j.fob.2013.09.005
Subject(s) - terminal (telecommunication) , rna , line (geometry) , chemistry , biochemistry , microbiology and biotechnology , biology , computer science , mathematics , gene , telecommunications , geometry
The human LINE‐1/L1 ORF2 protein is a multifunctional enzyme which plays a vital role in the life cycle of the human L1 retrotransposon. The protein consists of an endonuclease domain, followed by a central reverse transcriptase domain and a carboxy‐terminal C‐domain with unknown function. Here, we explore the nucleic acid binding properties of the 180‐amino acid carboxy‐terminal segment (CTS) of the human L1 ORF2p in vitro . In a series of experiments involving gel shift assay, we demonstrate that the CTS of L1 ORF2p binds RNA in non‐sequence‐specific manner. Finally, we report that mutations destroying the putative Zn‐knuckle structure of the protein do not significantly affect the level of RNA binding and discuss the possible functional role of the CTS in L1 retrotransposition.