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Two studies were conducted to evaluate the effect of nano chromium picolinate (nCrPic) during heat stress (HS) in sheep. In the initial study, 36 Merino × Poll cross-bred sheep were individually penned and allocated to 3 dietary treatments (0, 400 and 800 μg/kg nCrPic) for 8 wk. Body composition was determined at the beginning and end of the experiment using dual energy X-ray absorptiometry. The sheep remained in their dietary groups but were then placed in metabolic cages and randomly allocated within the dietary group to differing ambient temperature regimes, i.e., thermo-neutral (TN) ( n  = 18) and HS ( n  = 18), for 3 wk. Dietary nCrPic had no effect on growth performance and body composition during the initial study conducted under TN conditions. Heat stress decreased average daily feed intake (ADFI) ( P =  0.002) whereas sheep under HS had reduced average daily gain (ADG) and indeed lost weight ( P &lt;  0.001). Dietary nCrPic increased both ADFI ( P =  0.041) and ADG ( P =  0.049) under both TH and HS conditions such that the performance of sheep receiving supplemental nCrPic and exposed to HS was similar to that of control sheep maintained under TN conditions. Heat stress increased rectal temperature ( P &lt;  0.001) and respiration rate ( P &lt;  0.001), particularly during the hottest parts of the day as indicated by interactions ( P &lt;  0.001) between time of day and thermal treatment. Rectal temperature was lower in sheep fed nCrPic ( P =  0.050), particularly under peak HS conditions during the afternoon as indicated by the interactions between dietary nCrPic and time of day ( P &lt;  0.001) and dietary nCrPic, thermal treatment and time of day ( P =  0.010). Similarly, respiration rate was lower in sheep fed nCrPic under peak HS conditions during the afternoon as indicated by the interactions between dietary nCrPic and thermal treatment ( P &lt;  0.001) and dietary nCrPic and time of day ( P =  0.030). In conclusion, dietary nCrPic can partially ameliorate the negative effects of HS as indicated by the maintenance of ADFI and decreased physiological responses, such as elevations in rectal temperature and respiration rate.

Dietary nano chromium picolinate can ameliorate some of the impacts of heat stress in cross-bred sheep