English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Bioethanol is an important oil substitute produced by the sugar fermentation process. To improve the efficiency of cellulase expression of  Saccharomyces cerevisiae , a eukaryotic expression vector harboring a single-enzyme-system-three-cellulase gene ( sestc ) was integrated into the  S. cerevisiae  genome by the protoplast method. Using PCR screening, RT-PCR, and “transparent circle” detection, several recombinant  S. cerevisiae  strains, capable of efficiently expressing the heterogeneous cellulase, were selected. The total activity of cellulase, endo-β-D-glucanase, exo-β-D-glucanase, and xylanase of the recombinant  S. cerevisiae  transformant (designated number 14) was 1.1, 378, 1.44, and 164 U ml −1 , respectively, which was 27.5-, 63-, 24-, and 19-fold higher than that of the wild-type strain. The concentration of ethanol produced by the engineered  S. cerevisiae  strain was 8.1 gl −1 , with wheat bran as the carbon source, under submerged conditions; this was 57.86-fold higher than that produced by the wild-type strain (0.14 gl −1 ).

3 biotech

Construction of recombinant sestc Saccharomyces cerevisiae for consolidated bioprocessing, cellulase characterization, and ethanol production by in situ fermentation