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Therapeutic potential of medicinal plants as a source of noble natural anti-oxidants and anti-microbial agents has been well recognised all across the globe. In this study, phenolic compounds, in vitro anti-oxidant activity and anti-microbial properties have been investigated in five Himalayan medicinal plants, (e.g.,  Acorus calamus ,  Habenaria intermedia ,  Hedychium spicatum ,  Roscoea procera  and  Valeriana jatamansi ) in different solvent systems.  R. procera  exhibited significantly ( p  < 0.05) higher phenolics; while  H. spicatum  was rich in flavonoids and  V. jatamansi  in anti-oxidant activity. Also,  R. procera  and  H. spicatum  were found rich in gallic acid;  V. jatamansi  in catechin, hydroxylbenzoic acid and caffeic acid and  H. intermedia  in hydroxyl benzoic acid. Solvent systems showed species specific response for extraction of total flavonoids and anti-oxidant activity. All the extracts were found effective against different bacterial and fungal strains in a dose dependent manner and maximum antimicrobial activity was found in  R. procera  as compared to other species. All the plant extracts showed greater activity against bacterial strains as compared to fungal strains. The results of this study suggest that extract of these species can be used as natural anti-oxidant to reduce free radical mediated disorders and as natural alternative for food preservation.

Anti-oxidant and anti-microbial properties of some ethno-therapeutically important medicinal plants of Indian Himalayan Region