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In this study, five  Chlorella species  ( Chlorella vulgaris ,  Chlorella minutissima ,  Chlorella pyrenoidosa ,  Chlorella  sp.1 and  Chlorella  sp.2) were grown in various nutrient medium including BG-11, BBM, Fog’s medium and M 4 N medium for the evolution of biomass and lipid production potential. Among the tested medium, BG-11 was found most economical and efficient medium for all  Chlorella  species. To see the impact of organic carbon sources on lipid production potential, all microalgae species were also cultured in selected medium (BG-11) with different organic carbon sources like glucose, glycerol, sodium acetate, and sucrose under mixotrophic condition. The results showed that all  Chlorella  species performs better under mixotrophic condition, but  Chlorella vulgaris  achieved maximum lipid productivity (3.5 folds higher) in glycerol supplemented culture medium than control medium among all species.   Electronic supplementary material  The online version of this article (doi:10.1007/s13205-016-0434-6) contains supplementary material, which is available to authorized users.

Impact of various media and organic carbon sources on biofuel production potential from Chlorella spp.