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In this study, we developed a real-time PCR assay for RHDV detection and quantitation. With this method, the distribution of RHDV in the internal organs and body fluids of infected rabbits was analyzed. The highest viral RNA load was found in the spleen, followed by that in the liver. The virus is shed mainly through the oral, nasal, and urethral routes. Through testing of clinical samples, we found that most farmed rabbits that had been immunized had low levels of RHDV in their tissues. These findings may be useful for further research on the pathogenic mechanism of RHDV and potential RHDV receptors in organs.

virologica sinica

Development of a SYBR-based real-time PCR to detect rabbit hemorrhagic disease virus (RHDV) and analyze its tissue distribution in experimentally infected rabbits