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Separation of tripalmitin from its hydrolysis products by simple isocratic reversed‐phase high‐performance liquid chromatography
Author(s) -
Hampson J. W.,
Foglia T. A.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0262-x
Subject(s) - tripalmitin , chromatography , chemistry , hydrolysis , acetic acid , chloroform , glyceride , high performance liquid chromatography , triolein , acetonitrile , phase (matter) , lipase , fatty acid , organic chemistry , enzyme
An isocratic reversed‐phase high‐performance liquid‐chromatographic method was developed for monitoring the lipase‐catalyzed hydrolysis of solid triglycerides in supercritical carbon dioxide. The reaction products obtained consist of free fatty acids, monoglycerides, diglycerides, and unreacted triglycerides. For the method, developed with tripalmitin, a mobile phase of acetonitrile/chloroform/acetic acid (50:50:1, vol/vol/vol), a C 18 column, and refractive index detection were used. Analysis time is 7 min. Response factors were determined for each neutral lipid class, which permitted quantitation of the hydrolysis product mixture.