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A direct method for regiospecific analysis of TAG using α‐MAG
Author(s) -
Turon F.,
Bachain P.,
Caro Y.,
Pina M.,
Graille J.
Publication year - 2002
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-002-0966-3
Subject(s) - composition (language) , butterfat , chemistry , lipidology , chromatography , magnesium , adulterant , analytical chemistry (journal) , clinical chemistry , organic chemistry , milk fat , biochemistry , philosophy , linguistics , linseed oil
An analytical procedure was developed for regiodistribution analysis of TAG using α‐MAG prepared by an ethyl magnesium bromide deacylation. In the present communication, the deacylation procedure is shown to lead to representative α‐MAG, allowing the composition of the native TAG in the α‐position to be determined directly. The composition in the β‐position can then be estimated from the composition of the α‐MAG and TAG according to the formula, 3×TAG‐2×α‐MAG. The estimates are superior to those obtained using the α,β‐DAG and Brockerhoff calculations as they come closer to the theoretical value and have smaller SD. The present procedure, first demonstrated on a synthetic TAG, was then successfully applied to the analysis of borage oil, milkfat, and tuna oil.