Open AccessEmbryonic MTHFR contributes to blastocyst development
Author(s) -
Hiroki Ishitani,
Shuntaro Ikeda,
Kai Egashira,
Miki Sugimoto,
Shinji Kume,
Naojiro Minami,
Takeshi Ohta
Publication year - 2020
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-020-01898-0
Subject(s) - blastocyst , methylenetetrahydrofolate reductase , gene knockdown , andrology , inner cell mass , biology , embryogenesis , embryonic stem cell , embryo , genetics , genotype , gene , medicine
Reduction in methylenetetrahydrofolate reductase (MTHFR) activity due to genetic variations in the MTHFR gene has been controversially implicated in subfertility in human in vitro fertilization. However, there is no direct gene-knockdown study of embryonic MTHFR to assess its involvement in mammalian preimplantation development. The purpose of this study is to investigate expression profiles and functional roles of MTHFR in bovine preimplantation development.